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Monoclonal antibodies (mAbs) production from established hybridoma cell lines can diminish over time due to the decrease of the number of mAb-secreting cells. In many cases, mAb production can be rescued by re-cloning the hybridoma cell line and selecting out new highly antibody-positive clones. The clone that exhibits the best growth characteristics and produces the highest antibody titer will be expanded and frozen. Learn more: Hybridoma Re-cloning